5O or 5-O may refer to:
- ASL Airlines France (IATA airline code: 5O)
 - 5-O-caffeoylquinic acid, see Chlorogenic acid
 - 5-O-(4-coumaroyl)-D-quinate 3'-monooxygenase
 - 5-O-Methylations
 - Cyanidin 3-O-rutinoside 5-O-glucosyltransferase
 - Anthocyanin 5-O-glucoside 6'''-O-malonyltransferase
 - a slang term for a Ford Mustang GT automobile with a 5.0 L engine
 - 5.0, 2010 album by Nelly
 
See also
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