| Tournament details | |
|---|---|
| Country |  Bosnia and Herzegovina | 
| Teams | 36 | 
| Defending champions | Željezničar | 
| Final positions | |
| Champions | Željezničar 2nd national cup title | 
| Runner-up | Sarajevo | 
2000–01 Bosnia and Herzegovina Football Cup was the seventh season of the Bosnia and Herzegovina's annual football cup, and a first season of the unified competition. The competition started on 2 December 2000 with the First Round and concluded on 15 June 2001 with the Final.
First round
Thirty-two teams entered in the First Round. The matches were played on 2 December 2000.
| Team 1 | Score | Team 2 | 
|---|---|---|
| Grude | 0–3 | Željezničar | 
| Blagaj | 4–5 | Velež | 
| Tomislav | 0–2 | Čelik Zenica | 
| Bosna Visoko | 0–1 | Široki Brijeg | 
| Odžak 102 | 2–1 | Troglav | 
| Drina Zvornik-Živinice | 0–3 | Ljubuški | 
| Iskra Bugojno | 1–2 | Posušje | 
| Mramor | 3–2 | Budućnost Banovići | 
| Travnik | 5–0 | Đerzelez | 
| Sloboda Tuzla | 5–0 | Čapljina | 
| Brotnjo | 0–3 | Sarajevo | 
| Zrinjski | 1–0 | Rudar Kakanj | 
| FK Vitez | 2–0 | Orašje | 
| Kiseljak | 3–0 | Jedinstvo Bihać | 
| Butmir | 0–5 | Olimpik | 
| Podgrmeč Sanski Most | 5–0 | Krajina Cazin | 
Second round
The 16 winners from the prior round enter this round. The first legs were played on 6 December and the second legs were played on 9 December 2000.
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Posušje | 2–2 (a) | Sloboda Tuzla | 1–0 | 0–2 | 
| FK Vitez | 1–1 (a) | Čelik Zenica | 1–1 | 0–0 | 
| Mramor | 3–6 | Olimpik | 2–1 | 1–5 | 
| Odžak 102 | 2–3 | Kiseljak | 1–2 | 1–1 | 
| Sarajevo | 4–1 | Zrinjski | 3–0 | 1–1 | 
| Podgrmeč Sanski Most | 1–2 | Travnik | 0–0 | 1–2 | 
| Ljubuški | 1–4 | Željeznićar | 0–1 | 1–3 | 
| Velež | 1–5 | Široki Brijeg | 1–1 | 0–4 | 
Intermediate round
On January 31, it was announced that 4 clubs from Srpska will join 8 clubs from the NSBIH to play off for a unified cup.
The first legs were played on 14 March and the second legs were played on 28 March 2001.
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Boksit Milići | 0–4 | Posušje | 0–0 | 0–4 | 
| Čelik Zenica | 4–4 (a) | Omladinac APB Banja Luka | 2–3 | 2–1 | 
| Radnik Bijeljina | 2–3 | Olimpik | 0–2 | 2–1 | 
| Sarajevo | 1–0 | Kozara Gradiška | 1–0 | 0–0 | 
Quarterfinals
The eight winners from the prior round enter this round. The first legs were played on 4 April and the second legs were played on 25 April 2001.
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Travnik | 0–5 | Posušje | 0–3 | 0–2 | 
| Široki Brijeg | 3–1 | Omladinac APB Banja Luka | 2–1 | 1–0 | 
| Sarajevo | 3–0 | Kiseljak | 3–0 | 0–0 | 
| Željezničar | 4–0 | Olimpik | 4–0 | 0–0 | 
Semifinals
The four winners from the prior round enter this round. The first legs will be played on 9 May and the second legs were played on 25 May 2001.
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Široki Brijeg | 1–3 | Željezničar | 0–1 | 1–2 | 
| Posušje | 2–4 | Sarajevo | 1–0 | 1–4 | 
Final
| Sarajevo | 2 – 3 | Željezničar | 
|---|---|---|
| Repuh  19' Šaranović  50' | Report | Adžem  43' Alilhodžić  59' Muharemović  78' |